Genome-Wide Identification, Characterization and Expression of UROS Gene in Yesso Scallop Patinopecten yessoensis
HANG Yunna, WANG Yiying, SUN Hongyan, FAN Zhiyue, WANG Xubo, TIAN Ying, HAO Zhenlin, MAO Junxia, CHANG Yaqing
Key Laboratory of Mariculture & Stock Enhancement in North China′s Sea, Ministry of Agriculture and Rural Affairs, College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
Abstract：In order to explore the molecular function of uroporphyrinogen Ⅲ synthetase (UROS) gene in shell color formation, and provide more clues for the mechanism reseach of shell color in molluscs, the genome-wide identification, sequence characterization and expression of the UROS gene were investigated in 2 years old Yesso scallop Patinopecten yessoensis with brown and white shell colors, an important aquaculture species in North China by bioinformatic and quantitative real-time PCR methods. A single-copy of UROS gene, named PyUROS gene, was identified in the whole genome of the Yesso scallop, with full length of 68 808 bp containing 9 exons and 8 introns, and encoding 298 amino acids. A conserved HemD domain was predicted in the PyUROS protein sequence, which confirmed the identification of PyUROS gene. PyUROS gene was ubiquitously expressed in all the adult tissues of the Yesso scallop, indicating that the PyUROS gene had an important role in life activities of the Yesso scallop. Significantly different expression levels of PyUROS gene were found among different mantle regions and between different shell color individuals, which indicated that the key function of PyUROS gene was involved in shell coloration in the Yesso scallop. The finding will provide valuable information for molecular mechanism research of shell coloration, and lay a theoretical foundation for genetic breeding of the shell color trait.
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2024, Vol. 43 
