Identification and Analysis of Spfox-1 Gene and Its Regulation-Related lncRNA in Mud Crab Scylla paramamosain
WANG Jinjunsi1, ZHONG Zhaowei1, ZHANG Ziping2, WANG Yilei1
1. Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen 361021, China; 2. College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract：In order to explore the role of Spfox-1 gene and lncRNA with targeting relationship in gonadal development and larval development of mud crab Scylla paramamosain, Spfox-1 sequences with a 1410 bp open reading frame were obtained from its mature male and female gonadal transcriptome. The protein encoded by this sequence contained an RRM domain, which further predicts the acquisition of an lncRNA HX26820 that may have a targeting relationship with Spfox-1. Multiple comparisons and phylogenetic tree analysis revealed that the RRM domain of Spfox-1 was highly conserved and closely related to that of other arthropods. Real-time fluorescent quantitative PCR results showed that Spfox-1 was expressed in various tissues/organs of mature mud crab to varying extent, with the maximal expression in the ovary and brain ganglion in male. There was significantly higher expression of Spfox-1 at the vitellogenesis stage than that at other stages of ovarian development during gonadal development, while during the development of the testis, the expression continued to be declined. The expression of Spfox-1 was shown to be gradually increased from zoea Ⅴ stage to larval Ⅰ stage during larval development. However, the expression of lncRNA HX26820 with targeted relationship with Spfox-1 continued to decline during ovarian development, with high expression in only Z5. The results indicate that Spfox-1 and lncRNA HX26820 may participate in the process of ovarian development and larval development. The overexpression experiment showed that lncRNA HX26820 inhibited the expression of Spfox-1, suggesting that it may have a negative regulatory effect on the function of Spfox-1 in gonadal and larval development. It is the first time to investigate the correlation of gene and lncRNA in a cell line in mud crab, which laid a certain foundation for the study of regulation mechanism of lncRNA in crustaceans.
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2024, Vol. 43 
