Key Laboratory of Germplasm Improvement and Fine Seed Breeding for Marine Aquatic Animals, Key Laboratory of Protection and Utilization of Aquatic Germplasm Resources, Ministry of Agriculture and Rural Affairs, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China
Abstract：In order to investigate the effect of seasonal variation on the expression of genes from Toll-like receptor (TLR) signaling pathway of sea cucumber Apostichopus japonicus, the transcriptional expression levels of major factors from TLR signaling pathway were detected in sea cucumber with body weight of (236±5) g sampled from a sea cucumber culture pond from introduce breeding center in Liaoning Ocean and Fisheries Science Research Institute in the middle of each month from January to December 2018 by qRT-PCR method, and the water environmental parameters in the pond were analyzed with handheld multiparameter meter for evaluation of the correlations between the seasonal variation of TLR-related genes expression levels and the environmental parameters by Pearson's correlation test. The transcriptional expression analysis showed that there were low expression levels of Toll, IRAK4, TAK, P105, Rel, TLR3, TBK and IRF8 genes in June, indicating that TLR pathway was provided with low response ability in June. The correlation analysis showed that there was significantly positive correlation between MyD88 and TRAF6 genes expression and dissolved oxygen concentration, significantly positive correlation between TAK and TBK genes expression and redox potential, significantly negative correlation between IRF8 gene expression and water temperature, significantly positive correlation between SARM gene expression and water temperature, and significantly positive correlation between IκKβ gene expression and pHmv. The finding implied that the environmental factors led to important and complicated effects on the expression of TLR-related factors in the sea cucumber, among which the redox potential was key environmental factor for both MyD88-dependent and -independent pathways, the dissolved oxygen and pHmv were key environmental factors for MyD88-dependent pathway, and temperature was key environmental factor for MyD88-independent pathway.
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2024, Vol. 43 
