Effects of Microplastics on Activity, Digestion and Antioxidation Capability of Onchidium reevesii
SUN Qirui1,2, ZHANG Jiwu2, JIANG Hongxing3, ZHANG Hu4, MA Zhihao1,2, QIU Ming2, QIAO Guo2, LI Qiang 2, ZHANG Mingming2
1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 2. School of Marine and Biological Engineering, Yancheng Institute of Technology, Yancheng 224051, China; 3. Rural Work Bureau, Nanxiashu Street, Wujin District, Changzhou 213166, China; 4. Jiangsu Institute of Marine Fisheries, Nantong 226007, China
Abstract：In order to probe the toxic effect of microplastics exposure on marine beach shellfish, Onchidium reevesii with body weight of (8.3±0.5) g was kept in a 70 cm×50 cm×40 cm plastic tank (90 individuals/tank), and then was exposed to the low microplastics exposure (0.03%, microplastic mass/sediment mass), medium exposure (0.06%), high exposure group (0.09%), and control group (without microplastics). The effects of exposure for 6 h, 24 h, and 48 h on the activity (crawling distance) of O. reevesii, and activities of digestive enzymes and antioxidant enzymes were analyzed. Results showed that weak activities of O. reevesii were obviously observed in acute exposure to microplastics. At 6 h, the activities of protease, amylase (AMS) and lipase (LPS) in stomach and hepatopancreas (P<0.05) were found to be increased in the lower exposure (0.03%) of microplastics, the activity of lipase (LPS) in the intestine was shown to be increased in the medium exposure (0.06%) , and to be decreased in the activity of AMS in the intestine in the higher exposure (0.09%) . For the antioxidation-related enzymyes, the activity of superoxide dismutase (SOD) was increased, catalase (CAT) decreased in the lower exposure, and the contents of malondialdehyde (MDA) increased in hepatopancreas and coelomic fluid (P<0.05) at 6-hour exposure in higher exposure. The findings showed that the acute exposure of microplastics affected activity, digestion and antioxidation capability of O. reevesii obviously, and that provide a certain reference value for the species protection of O. reevesii.
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2024, Vol. 43 
