Comparative Transcriptome on Chinese Mitten Crab Eriocheir siensis with Aggressive Differences
XIE Xinyang, ZHOU Zhenqi, GUAN Weiye, HOU Xin, WANG Jun, WANG Chenghui
Shanghai Aquaculture Engineering and Technology Research Center, National Experimental Teaching Demonstration Center of Aquatic Science, Key Laboratory of Freshwater Aquatic Germplasm Resources Certificated by the Ministry of Agriculture and Rural Affairs,Shanghai 201306, China
Abstract：In order to explore the molecular basis of the differences in aggression behavior of Chinese mitten crab Eriocheir sinensis, comparative transcriptome analysis was conducted on the thoracic ganglia of the two groups of Chinese mitten crab with distinct aggressive differences. When the test crab claw was stimulated with a 5 mm in diameter and 40 cm long iron rod, the crab pinching or pushing away the iron rod with the chela was recorded as an attack, while it was not recordeded when fleeing or standing still in the face of the iron rod stimulation. A crab with an average number of attacks in three rounds of tests greater than or equal to the mean stimulus (5 times) was a strong attack crab, and vice versa. The results showed that a total of 3999 differentially expressed genes were identified between the strong and weak aggressive groups, in which 1556 genes were up-regulated and 2443 genes were down-regulated in the strong aggressive group. The up-regulated genes in the strong aggressive group were mainly enriched in cAMP signaling and RAS signaling pathways. The down-regulated genes were mainly enriched in growth-related pathways including tryptophan metabolism and carbohydrate metabolism. Meanwhile, the results pointed out that the 5-HT receptor genes were involved in the molecular regulation of aggressive behavior in Chinese mitten crab, and the expression levels of 5-HT receptor gene, 5-HT2BR, in the pectoralis ganglion and chelopods muscle were higher in the weak aggressive group than that of the strong aggressive group. However, there were lower expression levels of 5-HT2AR and 5-HT2BR in hemolymph and hepatopancreas than those in the aggressive group. The findings indicated that the strong aggressive behaviour of Chinese mitten crab would have more active nerve signal transduction ability, and that 5-HT receptor genes played roles in regulating aggressive behavior of Chinese mitten crab.
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2024, Vol. 43 
