Response of Hippo Pathway to Intestinal Injury Recovery in Pacific White Shrimp Litopenaeus vannamei Exposed to Ammonia Nitrogen and Cadmium Stresses
LIAO Guowei1, YU Jiaoping1, WANG Wanqi1, FAN Lanfen1,2
1. University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; 2. Research Center for Green Development of Agriculture, South China Agricultural University, Guangzhou 510642, China
Abstract：In order to explore the response of Hippo signaling pathway to intestinal injury recovery in Pacific white shrimp Litopenaeus vannamei under single-factor ammonia nitrogen or cadmium stress, the gene expressions of Hippo signaling pathway was examined in intestinal tissues of Pacific white shrimp with body weight of (3.99±0.50) g exposed to ammonia nitrogen or cadmiumstresses at 0 h, 24 h and 48 h during the stress phase, and at 3 h, 6 h, 12 h, 24 h and 48 h during the recovery phase, and intestinal histology was observed at the same time. The results showed that there was very significantly higher relative expression levels of Wts, Yki and TEAD genes at 6 h in the recovery stage after ammonia nitrogen stress compared with the control group (P<0.01), with significantly higher expression level of TEAD gene at 12 h in the recovery stage (P<0.05) and without significant difference in Hpo gene expression level, and the other stages was very significantly lower (P<0.01) in the recovery stage except for 3 h. Wts gene was found to be very significantly lower at 3 h and 24 h in the recovery stage (P<0.01) and significantly lower at 12 h and 48 h in the recovery stage (P<0.05). Wts gene was significantly lower at 24 h and 48 h in the shrimp exposed to cadmium stress compared with the control group (P<0.01). There were very significantly lower Hpo and Wts genes expression levels in the shrimp exposed to cadmium stress (P<0.01) except for Hpo gene at 6 h in the recovery stage and Wts gene at 48 h in the recovery stage. Yki gene was very significantly lower at 6 h, 12 h and 24 h in the recovery stage (P<0.01); TEAD gene was very significantly higher at 6 h in the recovery stage (P<0.01). Intestinal histological observation showed that the intestinal villus of the shrimp was completely shed at 48 h in the ammonia-nitrogen stress stage and 24 h in the cadmium stress stage, and that the intestinal villi showed obvious signs of recovery and growth at 24 h in the recovery stage. The findings indicated that the Hippo signaling pathway was involved in the regulation of the recovery and regeneration of intestinal villus under the single-factor stress of ammonia nitrogen and cadmium in Pacific white shrimp.
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2024, Vol. 43 
