Growth,Antioxidant Capability, and Immunity of Blunt Snout Bream Megalobrama amblycephala Juveniles under Chronic Ammonia Nitrogen Stress
LI Mengjiao, WANG Qian, ZHANG Ting, REN Jinliang, WANG Zhiyuan, ZHAO Weilan, WANG Hengjie, WANG Canli, YUAN Xiangyang
Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China
Abstract：In order to explore the effects of chronic ammonia nitrogen stress on the growth, liver antioxidant capacity and plasma immunity of blunt snout bream Megalobrama amblycephala juveniles, blunt snout bream with body weight of (30.85±1.21) g was raised in indoor culture barrels (120 cm high, 80 cm diameter) at water temperature of 22—28 ℃, and exposed to ammonium chloride concentrations of 0 (tap water, control group), and ammonium chloride of 11.09 mg/L (20% median-lethal concentration) for 56 days, based on the 96 h median-lethal concentration (LC50) of ammonia nitrogen solution on juvenile bream of 55.45 mg/L in the pre-test, with four replicates with 15 fish per replicate to evaluate the effects of ammonia nitrogen stress on growth, liver antioxidant capability and plasma immunity of juvenile blunt snout bream. The immune and antioxidant parameters were measured at the end of the feeding trial. The results showed that there were significantly higher weight gain and survival rate in control group than that of fish in stress group (P<0.05). Meanwhile, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were found to be significantly higher in liver of fish in control group than that of fish in stress group (P<0.05), without significant effects of ammonia nitrogen stress on AST, ALT and lactate dehydrogenase (LDH) activities in plasma of fish (P>0.05) compared to the control group. Superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) activities of fish were significantly higher in control group than those of fish in stress group (P<0.05), with decrease in malondialdehyde (MDA) level. Besides, ammonia nitrogen stress led to significantly decrease lysozyme, and acid phosphatase activities and albumin level in stress group (P<0.05) compared to the control group. In conclusion, ammonia nitrogen stress led to decrease growth, antioxidant capacity and innate immunity of the fish.
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2024, Vol. 43 
