Optimization of Purification Process of Saponins from Sea Cucumber Apostichopus japonicus with Macroporous Resin by Response Surface Methodology
LIU Yujun1, LIU Guiying1, YU Di1,2, LI Long1, FU Zhiyu1,2, ZHENG Jie1,2, LIU Yutong3, ZHOU Zunchun1,2
1. Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China; 2. Key Laboratory of Protection and Utilization of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs, Dalian 116023, China; 3. College of Chemistry, Liaoning University, Shenyang 110036, China
Abstract：In order to optimize the optimum process conditions for purifying saponin Holotoxin A1 (HA1) by macroporous resin, four different structural macroporous resins (AB-8, HP-20, D101 and NKA-9) commonly used for purification of saponins in sea cucumber Apostichopus japonicus were selected. The content of HA1 was determined by high performance liquid chromatography (HPLC) using sea cucumber saponin HA1 as a standard. By comparing their static adsorption-desorption capacity and static adsorption curves, the suitable resin type was selected. The crude saponin mass concentration, ethanol volume fraction and ethanol washing volume were used as factors to determine the optimal dynamic purification process conditions based on the single factor experiments and Box-Behnken response surface experiment. The results showed that AB-8 resin had the best purification effect on HA1, and the optimal purification process was as follows: crude saponin mass concentration of 25 mg/mL, flow rate 1 mL/min, sample loading volume 25 mL, washing volume 2 times column volume (BV), ethanol volume fraction 73%, and ethanol washing volume 7 BV. Under these conditions, the recovery rate of HA1 was 91.40%, and the purity was increased from 0.16% to 0.58%. The purification process obtained in this study is suitable for the purification of saponin HA1 in Apostichopus japonicus.
刘煜珺, 刘桂英, 于笛, 李龙, 傅志宇, 郑杰, 刘俣彤, 周遵春. 响应面法优化大孔树脂纯化仿刺参中皂苷工艺研究[J]. 水产科学, 2024, 43(5): 727-736.
LIU Yujun, LIU Guiying, YU Di, LI Long, FU Zhiyu, ZHENG Jie, LIU Yutong, ZHOU Zunchun. Optimization of Purification Process of Saponins from Sea Cucumber Apostichopus japonicus with Macroporous Resin by Response Surface Methodology. Fisheries Science, 2024, 43(5): 727-736.
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2024, Vol. 43 
