1. School of Life Sciences, Huzhou Normal University, Huzhou 313000, China; 2. Fishery Machinery and Instrument Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200092, China
Abstract：In order to evaluate the inhibitory effect of EM bacteria on cyanobacteria, EM bacteria including Lactobacillus plantarum, Acetobacter tropicalis, and Bacillus velezensis solution with total density of viable bacteria of 1×108 cfu/L were added to the cyanobacteria including Microcystis(content>95%), Anabaena, Oscillatoria and Spirulina solution at a dose of 0%(control group), 0.40%, 0.80%, 1.60% and 3.20% (volume fraction), and the concentrations of dissolved total nitrogen, dissolved total phosphorus, and dissolved organic carbon, pH, cyanobacteria density and total chlorophyll a level in the solution were determined at different culture periods. The results showed that adding 0.80%, 1.60% and 3.20% EM bacterial solution led to significantly reduce the concentration of total dissolved nitrogen in solution (P<0.05). The concentration of dissolved total phosphorus in the solution was shown to be significantly decreased by adding 3.20% of EM bacterial solution in the first 3 days (P<0.05), with little effect on the concentration of dissolved organic carbon in the final solution. There was stable pH in the solution containing EM bacteria. EM liquid was found to be significant inhibitory effect on cyanobacteria (P<0.01), with inhibitory rate of up to 90.00%, and the order of inhibitory effect as 0.80%>3.20%>1.60%>0.40%. The concentration of total chlorophyll a in solution was decreased by adding EM bacterial solution, with the descending order effects of inhibitory effect were as 0.80%>1.60%>0.40%>3.20%, 0.80% and significant effects on the concentration of total chlorophyll a in solution in 1.60% of EM bacterial solution (P<0.05).In summary, the best effect on inhibiting cyanobacteria and reducing total chlorophyll a was observed in 0.80% EM bacterial solution which was used to control cyanobacteria bloom in natural water or aquaculture water. The finding provides reference for controlling cyanobacteria bloom outbreak and regulating algal phase structure in aquaculture water.
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2024, Vol. 43 
