Effects of Salicylic Acid on High Temperature Resistance of Economic Brown Alga Sargassum thunbergii
WANG Xiaowei1, LYU Fang2,3,4, ZHOU Gefei1, WU Haiyi2,3,4
1. School of Life Science, YanTai University, Yantai 264005, China; 2. Shandong Province Macroalgae Resources Conservation and Application Engineering Research Center, Marine Science Institute of Shandong Province, Qingdao 266104, China; 3. Qingdao Key Laboratory of Coastal Ecological Restoration and Security, Qingdao 266104, China; 4. Qingdao Macroalgae Engineering Technology Research Center, Qingdao 266104, China
Abstract：In order to investigate the response of brown alga Sargassum thunbergii to high temperature stress and the effect of salicylic acid on its resistance to high temperature stress, four wild alga strains were placed in a 1000 mL conical flask, and 0, 1, 5, and 50 mg/L salicylic acid (SA) were added at 30 ℃ (treatment group), respectively. The growth rate, photosynthetic pigment content, chlorophyll fluorescence parameters of the alga strain were determined and the antioxidant system and HSP70 gene expression was detected after being cultured at high temperature for 3 days at 15 ℃ (control group) and 30 ℃ at different concentrations.The results showed that SA treatment significantly promoted the growth of the brown alga under high temperature. There were increase first and then decrease in specific growth rate, and chlorophyll a, chlorophyll c and carotenoid contents with elevated SA concentration, with the the most significant growth promotion in 5 mg/L SA group, with 589% increase in specific growth rate compared to the high temperature treatment, increase in the chlorophyll fluorescence parameter Fv/Fm, rebounded in non-photochemical quenching (NPQ) significantly, and increase in the activities of superoxide dismutase(SOD) and catalase (CAT). The expression level of HSP70 gene was found to be significantly increased under high temperature, 1 mg/L SA further promoting the expression of the HSP70 gene, whereas 5 mg/L and 50 mg/L SA leading to reduce HSP70 gene expression. The findings provide theoretical reference with the study of large-scale artificial breeding and mechanisms of stress resistance in S. thunbergii.
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2024, Vol. 43 
