Effects of Two Species of Green Microalgae on Water Quality and Biofilm Flora Structure in Aquaculture Systems of Pacific White Shrimp Litopenaeus vannamei
LYU Libin, LIU Chao, SUN Mingzhu, XU Ailing, SONG Zhiwen
Key Laboratory of Eco-Environmental Engineer and Pollution Remediation in Shandong Province, School of Environmental and Municipal Engineering, Qingdao Technological University, Qingdao 266000, China
Abstract：In order to investigate the effects of addition of green algae Chlorella pyrenoidosa and Haematococcus pluvialis on water quality, growth, biofilm bacterial community structure, and diversity of the Pacific white shrimp Litopenaeus vannamei in an internal circulation aquaculture system, a hydrophilic polyurethane gel filler with hanging film was loaded into a mesh bag, placed in an 80 L glass tank at 5% (V/V), with a salinity of 15 artificial seawater, and 64 juvenile shrimp (800 individuals/m3) with body length of (0.8±0.1) cm were stocked, and reared for 84 d and 10 mL of C. pyrenoidosa and H. pluvialis were added every 3 days, respectively, and no algae were added to the control group at water temperature of 26—28 ℃. Wash the hydrophilic polyurethane gel filler every 7 days and replenish it regularly. The results showed that the addition of microalgae led to reduce contents of nitrate nitrogen (NO-3-N) and total phosphorus (TP) in the aquaculture system, with decrease in NO3--N and TP by 13.0% and 17.7% in C. pyrenoidosa group and 6.3% and 29.7% H. pluvialis group. However, there were variations in ammonia nitrogen (TAN)and nitrite nitrogen (NO-2-N) performance: decrease in concentration in H. pluvialis group and increase in C. pyrenoidosa group in the aquaculture system. The body length, body weight, average daily growth rate (ADG), and feed utilization efficiency were improved in the shrimp exposed to addition of microalgae, without significant effect on shrimp survival rate. Body weight was found to be increased in the shrimp by 15.0% in C. pyrenoidosa group and 11.8% in H. pluvialis group.Analysis of bacterial community structure and diversity revealed that the relative abundances of Proteobacteria and Bacteroidetes were decreased in C. pyrenoidosa system and the relative abundance of Acidobacteria was increased; relative abundance of Bacteroidetes was increased in H. pluvialis system while Acidobacteria and Proteobacteria were decreased instead. Additionally, the relative abundance of nitrifying bacteria was shown to be increased in the added microalgae culture systems, while the relative abundance of denitrifying bacteria to be decreased.
吕丽斌, 刘超, 孙明珠, 徐爱玲, 宋志文. 两种微藻对凡纳滨对虾养殖系统水质与生物膜菌群结构的影响[J]. 水产科学, 2025, 44(2): 223-234.
LYU Libin, LIU Chao, SUN Mingzhu, XU Ailing, SONG Zhiwen. Effects of Two Species of Green Microalgae on Water Quality and Biofilm Flora Structure in Aquaculture Systems of Pacific White Shrimp Litopenaeus vannamei. Fisheries Science, 2025, 44(2): 223-234.
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