Abstract：In order to improve the accuracy of kelp Saccharina japonica preservation, a method for constructing the core breeding populations of the kelp with diverse phenotypes and comprehensive genetic informationwas proposed for the first time by utilizing the microsatellite molecular markers of the kelp, and applied in four distinct lines. A total of 153 SSR markers from the kelp microsatellite marker library were used in the DFKZ-3,DFXZ-3,DFXZ-1,and DFXZ-2 breeding populations and 15 loci with good polymorphism were screened out from each line to analyze the genetic diversity of the population. After a comprehensive evaluation of allelic weight scores, individual combination scores, species status, and other relevant factors, the final number of preserved individuals in the DFKZ-3, DFXZ-3, DFXZ-1, and DFXZ-2 kelp lines was determined as 12, 12, 11, and 17, respectively. Germplasm collection and separation were carried out when the sporangium was mature according to the number of the preserved kelp, and gametophytes were retained for seed preservation, so as to build a core breeding population, which laid a foundation for the sustainable utilization of improved varieties, further development of germplasm resources and screening of more core kelp seed banks.
李言, 刘延岭, 梁广津, 王利芹, 李晓捷, 赵聚萍, 武瑞娜, 田萍萍. 基于SSR标记构建海带核心繁育群体[J]. 水产科学, 2025, 44(5): 717-726.
LI Yan, LIU Yanling, LIANG Guangjin, WANG Liqin, LI Xiaojie, ZHAO Juping, WU Ruina, TIAN Pingping. Construction of Different Core Breeding Populations of Kelp Saccharina japonica Based on SSR Markers. Fisheries Science, 2025, 44(5): 717-726.
[1] 张全胜,罗世菊.海带种质保存技术的研究现状和发展前景[J]. 水产科技情报,2006,33(2):61-63. [2] 刘涛,崔竞进,戴继勋,等.海带配子体克隆的培养及应用[J]. 中国海洋大学学报(自然科学版),2000,30(增刊):203-206. [3] 张全胜.海带种质保存技术[J]. 中国水产,2005(9):64-65. [4] 崔翠菊,李言,刘延岭,等.运用分子标记辅助构建海带核心种质资源[J]. 海洋科学,2016,40(1):18-24. [5] 戴继勋.用海藻细胞工程技术,发展我国的海水养殖业[J]. 世界科技研究与发展,2002,24(3):28-31. [6] 杨官品,李晓捷,丛义周,等.海带配子体无性繁殖(克隆)技术研究与应用进展[J]. 中国海洋大学学报(自然科学版),2007,37(4):569-572. [7] 王飞久,刘涛,段德麟,等.海带种质特征[J]. 海洋水产研究,2004,25(1):48-51. [8] 张全胜,石媛媛,丛义周,等.我国引种海带和栽培品种(系)来源配子体克隆的AFLP分析[J]. 中国海洋大学学报(自然科学版),2008,38(3):429-435. [9] WANG X L, YANG Y X, CONG Y Z, et al.DNA fingerprinting of selected Laminaria (Phaeophyta) gametophytes by RAPD markers[J]. Aquaculture,2004,238(1/2/3/4):143-153. [10] WANG X L, LIU C L, LI X J, et al. Assessment of genetic diversities of selected Laminaria (Laminariales, Phaeophyta) gametophytes by inter-simple sequence repeat analysis[J]. Journal of Integrative Plant Biology,2005,47(6):753-758. [11] LI X J, YANG G P, SHI Y Y, et al. Prediction of the heterosis of Laminaria hybrids with the genetic distance between their parental gametophyte clones[J]. Journal of Applied Phycology,2008,20(6):1097-1102. [12] 石媛嫄,杨官品,廖梅杰,等.海带和长海带配子体无性繁殖系微卫星DNA多态性比较分析[J]. 中国海洋大学学报(自然科学版),2008,38(2):303-308. [13] LI Y C, KOROL A B, FAHIMA T, et al. Microsatellites:genomic distribution, putative functions and mutational mechanisms:a review[J]. Molecular Ecology,2002,11(12):2453-2465. [14] TÓTH G, GÁSPÁRI Z, JURKA J. Microsatellites in different eukaryotic genomes:survey and analysis[J]. Genome Research,2000,10(7):967-981. [15] ZHANG L N, PENG J, LI X J, et al. Development of 27 trinucleotide microsatellite markers for Saccharina japonica using next generation sequencing technology[J]. Conservation Genetics Resources,2014,6(2):341-344. [16] PENG J, ZHANG L N, LI X J, et al. Development of genic SSR markers from an assembled Saccharina japonica genome[J]. Journal of Applied Phycology,2016,28(4):2479-2484. [17] 彭捷,崔翠菊,梁广津,等.基于SSR标记的海带、裙带菜的遗传构成分析[J]. 生物技术通报,2018,34(3):156-162. [18] 刘向宇,霍秀文,杨明,等.山药种质资源的ISSR分析及初级核心种质库的构建[J]. 西北植物学报,2015,35(5):915-921. [19] 李丽,何伟明,马连平,等.用EST-SSR分子标记技术构建大白菜核心种质及其指纹图谱库[J]. 基因组学与应用生物学,2009,28(1):76-88. [20] 齐永文,樊丽娜,罗青文,等.甘蔗细茎野生种核心种质构建[J]. 作物学报,2013,39(4):649-656. [21] 刘遵春,刘大亮,崔美,等.整合农艺性状和分子标记数据构建新疆野苹果核心种质[J]. 园艺学报,2012,39(6):1045-1054. [22] LIU F L, WANG X L, YAO J T, et al. Development of expressed sequence tag-derived microsatellite markers for Saccharina (Laminaria) japonica[J]. Journal of Applied Phycology,2010,22(1):109-111. [23] LIU F L, YAO J T, WANG X L, et al. Genetic diversity and structure within and between wild and cultivated Saccharina japonica (Laminariales, Phaeophyta) revealed by SSR markers[J]. Aquaculture,2012,358:139-145. [24] SHAN T F, PANG S J, ZHANG Y R, et al. An AFLP-based survey of genetic diversity and relationships of major farmed cultivars and geographically isolated wild populations of Saccharina japonica (Phaeophyta) along the northwest coasts of the Pacific[J]. Journal of Applied Phycology,2011,23(1):35-45.
2025, Vol. 44 
