Abstract：To understand the effects of different diets on ovarian development and reproductive performance of Acrossocheilius hemispinus brood fish, the broodstock with an initial body weight of about 120 g were reared in a glass fiber reinforced plastic barrel with an inner diameter of 3.0 m, a height of 1.2 m and a water depth of 0.7—0.8 m and fed ordinary feed, water tubifex Limnodrilus hoffmeisteri and intensified diet for 30 d at water temperature of 22.0—28.5 ℃. Then the ovarian sections were histologically observed and counted, the gonado-somatic index and the ratio of developmental stage Ⅳ were calculated and the contents of crude protein, crude fat, crude ash, phosphorus and calcium and moisture were determined in ovary of A. hemispinus, with statistics of success rate, fertilization rate, and hatching rate during the artificial reproduction. There was gonado-somatic index of 9.82% in water tubifex group and 9.65% in intensified diet group, significantly higher than that in the control group (8.20%) (P<0.05). The ratio of developmental stage Ⅳ was found to be 33.16% in water tubifex group and 32.38% in intensified diet group, significantly higher than that in the control group (27.85%) (P<0.05). The significant increase in moisture was observed in ovarian in water tubifex group and intensified diet group (P<0.05), and significant increase in crude fat and phosphorus contents (P<0.05), without significant difference in the contents of crude protein, crude ash and calcium (P>0.05). There was success rate of 53.50% in ordinary feed group, 52.46% in water tubifex group and 54.28% in intensified diet group, without significant difference among the three groups (P>0.05), with significant increase in fertilization rate by 11.95% in water tubifex group and 13.74% in intensified diet group compared with that in ordinary feed group. The hatching rate was shown to be slightly higher in water tubifex group and intensified diet group than that in ordinary feed group (P>0.05). Comprehensive analysis of ovarian development and reproductive performance and other factors, feeding self-made intensified diet or worm can improve the ovarian development and reproductive performance of A. hemispinus.
[1] 《福建鱼类志》编写组.福建鱼类志(上) (1984)[M]. 福州:福建科学技术出版社,1984:337-338. [2] 陈度煌,张天华,薛凌展,等.饵料中添加藻粉对半刺厚唇鱼消化酶活性、抗氧化能力及体成分的影响[J]. 饲料研究,2022,45(21):67-69. [3] 陈度煌,林而舒,薛凌展,等.2种土著淡水鱼亲鱼强化研究进展与展望[J]. 中国农学通报,2022,38(29):146-151. [4] 秦志清,樊海平,薛凌展,等.半刺厚唇鱼嗜酸性卵甲藻病的防治[J]. 科学养鱼,2017(4):64-65. [5] 张晶晶.半刺厚唇鱼源维氏气单胞菌的分离鉴定[J]. 中国农学通报,2023,39(20):138-146. [6] 仲崇虎.半刺厚唇鱼雌雄形态差异和胚胎发育研究[D]. 南京:南京农业大学,2022. [7] 秦志清,樊海平,薛凌展,等.半刺厚唇鱼的耗氧率与窒息点[J]. 福建农业学报,2017,32(10):1072-1076. [8] 刘丽丽,郑欣欣,尤永隆,等.半刺厚唇鱼的人工繁殖试验[J]. 淡水渔业,2010,40(2):57-61. [9] 秦志清,樊海平,林建斌,等.土池主养半刺厚唇鱼关键技术及效果研究[J]. 福建农业科技,2019,50(8):46-48. [10] 李传武,梁志强,伍远安,等.半刺厚唇鱼肌肉营养成分分析[J]. 营养学报,2011,33(5):526-528. [11] 林建斌,梁萍,秦志清,等.野生与养殖半刺厚唇鱼肌肉营养成分比较分析[J]. 中国饲料,2017(22):27-30. [12] HOANG T, LEE S Y, KEENAN C P, et al. Ovarian maturation of the banana prawn, Penaeus merguiensis de Man under different light intensities[J]. Aquaculture,2002,208(1/2):159-168. [13] MYLONAS C C, FOSTIER A, ZANUY S.Broodstock management and hormonal manipulations of fish reproduction[J]. General and Comparative Endocrinology,2010,165(3):516-534. [14] CHATZIFOTIS S, PAPADAKI M, DESPOTI S, et al. Effect of starvation and re-feeding on reproductive indices, body weight, plasma metabolites and oxidative enzymes of sea bass (Dicentrarchus labrax)[J]. Aquaculture,2011,316(1/2/3/4):53-59. [15] 李亚宁.小麦胚芽对黄颡鱼雌性亲鱼健康及繁殖性能的影响[D]. 武汉:华中农业大学,2022. [16] 龙勇,李芹,罗莉,等.饲料蛋白水平对异育银鲫雌性性腺发育的影响[J]. 水生生物学报,2008,32(4):551-557. [17] MASRIZAL, UDIN Z, ZEIN M, et al. Effect of energy, lipid and protein content in broodstock diets on spawning fecundity and eggs quality of giant gourami (Ospheronemus gouramy lac)[J]. Pakistan Journal of Nutrition,2015,14(7):412-416. [18] 黄利娜,梁萌青,张海涛,等.饲料中添加不同水平维生素A对大菱鲆亲鱼繁殖性能的影响[J]. 渔业科学进展,2013,34(4):62-70. [19] 张海涛.饲料中维生素C、维生素E与维生素C联用对大菱鲆亲鱼繁殖性能的影响[D]. 青岛:中国海洋大学,2012. [20] 李铁梁,邢薇,徐冠玲,等.维生素C对龙睛金鱼亲鱼繁殖性能、免疫及后代仔鱼质量的影响[J]. 大连海洋大学学报,2022,37(5):732-738. [21] WIEGAND M D. Composition, accumulation and utilization of yolk lipids in teleost fish[J]. Reviews in Fish Biology and Fisheries,1996,6(3):259-286. [22] SEIFI BERENJESTANAKI S, ESMAEILI FEREIDOUNI A, OURAJI H, et al. Influence of dietary lipid sources on growth, reproductive performance and fatty acid compositions of muscle and egg in three-spot gourami (Trichopodus trichopterus) (Pallas,1770)[J]. Aquaculture Nutrition,2014,20(5):494-504. [23] 马爱军,雷霁霖,王新安,等.大菱鲆亲鱼、配子和仔稚鱼的质量评价[J]. 海洋科学,2011,35(1):98-104. [24] 倪子绵,张其永,洪万树.大弹涂鱼卵细胞发育的显微和超微结构[J]. 台湾海峡,1995(2):163-168. [25] 焦圣博,何牡丹,孙永华.鱼类卵子质量研究及其关键科学问题[J]. 水生生物学报,2023,47(6):1007-1024.
2025, Vol. 44 
