Effect of Salinity on Alkaline Potassium Persulfate Oxidation Method for Determination of Total Nitrogen Analysis
YIN Zhijian, JIANG Chengcheng, LIU Jianlong, WANG Shiwei, WANG Lianshun, CONG Yuting, WANG Li, WANG Hua, WANG Yuan, LU Yanan, YANG Guojun
1. National Demonstration Center for Experimental Aquaculture Education, Ministry of Education, College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 2. Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University), Ministry of Education, Dalian 116023, China; 3. Dalian Key Laboratory of Breeding, Reproduction and Aquaculture of Crustaceans, Dalian 116023, China
Abstract：This study systematically explored the applicable salinity range of seawater total nitrogen analysis method [alkaline potassium persulfate oxidation method (GB 17378.4—2007)] and water quality total nitrogen analysis method [alkaline potassium persulfate oxidation-ultraviolet spectrophotometry (HJ 636—2012)] and the reason why water quality total nitrogen analysis method could not test high salinity samples. In the test, the applicable salinity range of the two total nitrogen analysis methods was determined by comparing the blank value, r2, and nitrate reduction rate of the eight different standard curves of the two water quality analysis methods in the salinity range from 0 to 35. The results showed that the seawater total nitrogen analysis method could detect seawater samples with different salinity gradients, and with the recovery rate from 92% to 100%. The total nitrogen analysis method can only detect seawater samples with salinity ≤20, with the recovery rate from 92% to 106%. At the salinity of over 20, there is potassium persulfate residue in the sample using the water quality total nitrogen analysis method. Subsequently, the potassium persulfate residual concentration of the total nitrogen analysis method of water quality at different salinities was compared by the potassium persulfate residual experiment and acetic acid titration experiment to verify why the potassium persulfate oxidation method could not determine high salinity. Finally, the total nitrogen analysis method of seawater and the total nitrogen analysis method of water quality are compared to detect seawater samples to determine whether the dilution method can detect seawater samples. In addition, the water quality total nitrogen analysis method cannot detect high-salinity seawater diluted samples. The finding systematically analyzes the applicable salinity range of the two total nitrogen analysis methods, explains why the water quality total nitrogen analysis method can not detect high-salinity samples, and provides a reference and basis for the detection of total nitrogen index and method improvement in the future.
尹智践, 姜程程, 刘健龙, 王世伟, 王连顺, 丛玉婷, 王丽, 王华, 王媛, 卢亚楠, 杨国军. 盐度对总氮碱性过硫酸钾氧化法的影响研究[J]. 水产科学, 2025, 44(5): 829-835.
YIN Zhijian, JIANG Chengcheng, LIU Jianlong, WANG Shiwei, WANG Lianshun, CONG Yuting, WANG Li, WANG Hua, WANG Yuan, LU Yanan, YANG Guojun. Effect of Salinity on Alkaline Potassium Persulfate Oxidation Method for Determination of Total Nitrogen Analysis. Fisheries Science, 2025, 44(5): 829-835.
[1] 付志茹,王娜,杨华,等.刺参“水院1号”幼参培育水质检测分析[J]. 天津农业科学,2016,22(12):99-105. [2] 王光玉,姜佳惠,祝楠,等.换水率和密度对刺参生长和水质的影响[J]. 渔业现代化,2017,44(6):55-61. [3] 于琪.基于海参养殖水体净化的益生菌筛选与应用[D]. 大连:大连理工大学,2019. [4] HERBECK L S, UNGER D, WU Y, et al. Effluent, nutrient and organic matter export from shrimp and fish ponds causing eutrophication in coastal and back-reef waters of NE Hainan, tropical China[J]. Continental Shelf Research,2013,57:92-104. [5] 夏丽华,徐珊,陈智斌,等.广东省海岸带海水养殖业污染贡献率研究[J]. 广州大学学报(自然科学版),2013,12(5):80-86. [6] CAO L, WANG W M, YANG Y, et al. Environmental impact of aquaculture and countermeasures to aquaculture pollution in China[J]. Environmental Science and Pollution Research International, 2007,14(7):452-462. [7] JOBLING M. Some effects of temperature, feeding and body weight on nitrogenous excretion in young plaice Pleuronectes platessa L[J]. Journal of Fish Biology,1981,18(1):87-96. [8] PORTER C B, KROM M D, ROBBINS M G, et al. Ammonia excretion and total N budget for gilthead seabream (Sparus aurata) and its effect on water quality conditions[J]. Aquaculture,1987,66(3/4):287-297. [9] 王东升,张金川,刘飏, 等.海洋系统中氮的生物地球化学循环回顾与展望[J]. 地球与环境,2023,51(4):465-481. [10] 国家质量监督检验检疫总局,中国国家标准化管理委员会.海洋监测规范 第4部分:海水分析:GB 17378.4—2007[S]. 北京:中国标准出版社,2008. [11] 乔晓平.过硫酸钾消解-离子色谱法测定地表水中总氮的研究[J]. 环境科学与管理,2015,40(3):127-129. [12] 陈颖,马琳.海水中总氮的测定方法比较研究[J]. 环境监控与预警,2018,10(4):32-36. [13] 车淑红,郭新莉,郎毅.咪唑缓冲溶液-连续流动测定总氮的方法改进[J]. 人民黄河,2021,43(增刊1):94-95. [14] 任朝兴,黄国娟,刘燕,等.气相分子吸收光谱法测定海水中总氮[J]. 分析仪器,2020(2):40-43. [15] 中华人民共和国环境保护部.水质 总氮的测定 碱性过硫酸钾消解紫外分光光度法:HJ 636—2012[S]. 北京:中国环境科学出版社,2012. [16] 侍爱秋.水中总氮准确测定的影响因素研究[J]. 化学工程与装备,2014(2):197-199. [17] 黄文国,吴碧玉,张光磊.海水总氮测定的影响因素及注意事项[J]. 广东化工,2011,38(8):141. [18] 黎文,白英臣,王立英,等.淡水湖泊水体中溶解有机氮测定方法的对比[J]. 湖泊科学,2006,18(1):63-68. [19] 欧登龙.过硫酸钾对总氮测定的影响与优化[J]. 广东化工,2016,43(11):266-267. [20] 曾海燕,危建新,王文明.实验用水对总氮检测结果的影响[J]. 四川环境,2014,33(6):102-104. [21] 谈锦清.总氮测定中消解容器的改进[J]. 中国环境监测,2002,18(5):63. [22] 郑秋妹.总磷总氮消解方法的改进分析[J]. 环境与发展,2019,31(9):120-121. [23] 李碧莹,马晓娜,田会芹,等.盐度对养殖水体中总氮总磷浓度测定的影响研究[J]. 海洋湖沼通报,2021,43(5):127-135. [24] 徐玉珊,刘新杰,张欣欣,等.过硫酸钾氧化法测定海水中总氮的影响因素和解决方法[J]. 化工设计通讯,2023,49(8):180-182. [25] 孙艳涛,李安琪,马金明,等.数字化方法测定食醋中醋酸含量教学设计[J]. 牡丹江大学学报,2017,26(12):133-135. [26] 潘忠成,李敏.HJ 636—2012测定总氮时影响空白值因素分析[J]. 环境工程,2016,34(1):126-129. [27] 王金叶,李朝霞,毛玉泽.锌-镉还原法测定天然水中硝酸盐的最优测定条件[J]. 大连海洋大学学报,2014,29(3):256-259. [28] 王燕,王艳洁,赵仕兰,等.海水中溶解态总氮测定方法比对及影响因素分析[J]. 海洋环境科学,2019,38(4):644-648. [29] 王中荣.总磷总氮测定仪的研制与优化[D]. 石家庄:河北科技大学,2016.
2025, Vol. 44 
