Abstract：25-Hydroxyvitamin D3 is the active metabolite of vitamin D3. In order to investigate the effects of dietary supplementation with 25-hydroxyvitamin D3 on the growth performance and hepatic biochemical parameters of yellow catfish Pelteobagrus fulvidraco, yellow catfish juveniles with initial body weight of (2.4±0.5) g were reared in glass tanks measuring 60 cm × 40 cm × 35 cm and fed diets supplemented with 0 (control), 1000, 2000, 4000, 8000, and 16 000 IU/kg 25-hydroxyvitamin D3 at water temperature of 22—28 ℃ for 56 days. The results showed that (1) there were significantly higher final body weight, final body length, weight gain rate, specific growth rate, and viscerosomatic ratio in the yellow catfish in 2000 IU/kg group compared to the control group, with significantly lower feed conversion ratio (P＜0.05). (2) Total cholesterol levels in the liver were shown to be significantly higher in 2000 IU/kg group than those in the control group, with significantly higher triglyceride content in the 4000 IU/kg group than that in the control group (P＜0.05). (3) The yellow catfish had significantly lower activities of aspartate aminotransferase in treatment groups than the yellow catfish did in the control group, with significantly higher acid phosphatase and alkaline phosphatase activities (P＜0.05). Significantly increase in lysozyme activity was observed in the 4000 IU/kg group (P＜0.05). (4) Compared with the control group, catalase activity was found to be significantly increased in the 4000 IU/kg group (P＜0.05), and total superoxide dismutase activity in the 2000 IU/kg group was significantly higher than that in the control group (P＜0.05), with significantly lower malondialdehyde level in the 4000 IU/kg group than that in the control group (P＜0.05). In summary, adding 2000 IU/kg or 4000 IU/kg of 25-hydroxyvitamin D3 to a basal diet containing 3 mg/kg of vitamin D3 can significantly improve the growth performance, hepatic immune function, and antioxidant capacity of yellow catfish. Based on the regression equations of weight gain rate and feed conversion ratio, the optimal supplementation level of 25-hydroxyvitamin D3 in the diet of yellow catfish was determined to be 2240 IU/kg.
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2026, Vol. 45 
