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| Isolation,Identification and Physiological Characterization of Lytic Phage against Bacterium Salmonella sp. |
| LI Meng,HAN Feng,LIN Hong,WANG Jing-xue |
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Abstract A lytic phage SP3 was isolated from effluent from a fishery product market by a double-layer plate method to develop an effective controlling method against bacterium Salmonella sp .The SP3 were morphologically observed by transmission electron microscope ,and the composition of protein and nucleic acid were analyzed by SDS-PAGE and AGE . Furthermore some physiological properties were also characterized including one step curve , pH stability , thermal stability , inhibition activity and storage condition .The SP3 was found to be a member of family Siphoviridae ,whose main proteins were ranged from 43 .0 to 66 .2 ku and the deoxyribose nucleic acid (DNA ) was digested with the restriction endonuclease .The latent time period and burst time were calculated to be 5 and 75 min ,respectively .The SP3 was stable at a wide range of temperatures from 30 to 40 ℃ and pH 7 ~ 9 . At liquid culture conditions ,the bacterium treated with phage had much lower optical density than the control one .The storage condition test revealed that the SP3 should be stored at -20 ℃ .
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Received: 25 September 2013
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