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| Physiological Response of Algae Py ramidomonas delicatula and Chlorella saccharophila to Glyphosate Toxicity Stress |
| TIAN Mi,ZHENG Beibei,SU Jinli,SONG Xiumei,WANG Hongbin |
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Abstract The physiological effects of herbicide glyphosate on growth and synthesis of chlorophyll a were studied in algae Pyramidomonas delicatula and Chlorella saccharophila to alert the pesticide pollution severity in water environment and to reveal potential application of marine microalgae in environmental toxicological evaluation . The results showed that the glyphosate had no inhibitory effect on the alga grow th within the concentration in all experimental groups in the first 3 days ,showing toxicity excitement at 10μL of glyphosate ,with the maximal value in 3 day ,39% higher than that in control group .From the day 4 , however , glyphosate showed significant inhibitory effect on P . delicatula and a clear dose-dependent relationship , up to the peak in 80 μL group 7 d after cultivation , the biomass in the experimental group representing 47% of the control group .Glyphosate was found to have stronger toxicity stress on C .saccharophila than on P .delicatula ,being increased with the increase in concentration and time elapse from the 2 d .In 7 day cultivation ,the biomass was found to be 27 .4% of the control group at 10 μL of glyphosate ,24 .6% at 20 μL of glyphosate ,23 .7% at 40 μL of glyphosate ,21 .9% at 60 μL of glyphosate ,and 13% at 80 μL of glyphosate .The glyphosate showed inhibitory effect on chlorophyll a of the algae ,the minimal value in 80 μL group ,and the content of chlorophyll a was only 24 .3% ( P . delicatula) and 7 .54% (C .saccharophila) of the control group .Glyphosate showed much more toxicity stress on C .saccharophila than on P . delicatula ,indicating the feasibility of C .saccharophila as the toxicological evaluation for glyphosate water pollution .
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Received: 25 September 2013
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