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| Lipid Content and Fatty acid Composition in Green Alga Chlorella py renoidesa at Different Growth Phases |
| CHEN Wei,LIANG Mingming,BAI Yongan,LI Xiaodong,ZHAO Wen |
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Abstract Alga Chlorella pyrenoidesa was cultured in the outdoor ponds at 25~28.5 ℃ for 18 days .The microalga were collected at different growth phases (delay phase ,exponential growth phase ,stationary phase and decay phase) .The effect of different growth phases on the total lipid content and the fatty acid composition of Chlorella pyrenoidesa were investigated .The fatty acids composition was determined by capillary gas-liquid chromatography .The results showed that the lipid content in the alga (4 .85% of fresh weight) gathering from exponential growth phase was significantly higher than those from the other three growth phases (1.44% ~1.85% of fresh weight ) .Maximum percentages of polyunsaturated fatty acid (PUFA ) and eicosapentaenoic acid (EPA ) were found in exponential growth phase ,followed by on stationary phase and delay phase ,and the minimum on decay phase .The percentage of PUFA and EPA were significantly decreased in stationary phase (17 .92% and 12 .98% ) , delay phase (17 .53% and 13 .02% ) ,and decay phase (12 .47% and 8 .62% ) relative to exponential phase (23 .37% and 17 .92% ) . Therefore ,late exponential growth phase or early stationary phase in the outdoor culture of Chlorella pyrenoidesa was the optimal harvesting phase .
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Received: 25 September 2013
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