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Abstract The fatty acid composition in muscle of fry and muscle ,hepatopancreas ,abdominal fat and ovary of female tilapia Oreochromis sp were analyzed during spawning season by chemical method and gas chromatograph .The results showed that there were rich in C16:0 ,C16:1 ,C18 :0 ,C18:1n-9 and C18:2n-6 , representing more than 78% in total fatty acid .Fatty acid content in tissues of the tilapia was varied with different growth stages and tissues of the fish .The fry had significantly lower muscular C18:2n-6 content (8 .13% ) than the females (12 .36% ~16 .27% )(P<0 .05)which had 12 .36% ~16 .27% of C18 :2 n-6 in muscle ,abdomen ,and ovary during spawning season(P<0 .05) .The fry had 3 .24% of C20 :4n-6 ,higher than in the other tissues of the females ,next to that in the ovary of the females in which the C20 :4n-6 contents were arranged in various tissues as :ovary > hepatopancreas > muscle> abdominal fat . The contents of C20 :5n-3 and C22 :6n-3 were found to be 0% in the fry ,with maximum (1 .46% and 4 .73% )in the ovary of the females (P< 0 .05) .Ratio of n-3/n-6 < 1 was observed in muscle of fry and muscle , hepatopancreas ,abdominal fat and ovary of the female .The various fatty acid contents were ranged asΣSFA> ΣMUFA> ΣPUFA in muscle of the fry and hepatopancreas and ovary of the females ,and ΣMUFA> ΣSFA> ΣPUFA in muscle and abdominal fat of the females during spawning season .The findings reveal that more C18 :2n-6 content is added into broodstock diet to improve the synthesis of unsaturated fatty acids and to promote gonadal development ,and more C20 :4n-6 content is found in larval diet to improve the juvenile quality .
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Received: 25 October 2013
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