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Abstract The hemolymph osmotic regulation was studied in adult mudflat crab ( Helice tientsinensis ) exposed to a salinity of 0 ,10 ,20 ,30 ,40 and 50 for 9 days at 20 °C in aquarium using vapor pressure osmometer .The results showed that there was lower hemolymph osmotic pressure in the mudflat crab exposed to the salinity from 30 to 0 ,higher than that in ambient osmotic pressure .The mudflat crab exposed to the salinity from 30 to 50 had higher hemolymph osmotic pressure ,lower than that in ambient osmotic pressure .The exposure period was found to affect the hemolymph osmotic pressure .There was significant difference in hemolymph osmotic pressure at a salinity of 0 ,10 ,and 50 except 20 ,30 and 40 (P< 0 .05) between 0 h and 3 h after the experiment .The mudflat crab had isosmotic point of 815 .6 mmol/kg ,equivalent to 27 .1 for salinity concentration .The findings indicated that the mudflat crab as an osmoregulator had good regulation of osmotic pressure ability at water temperature of 20 ℃ .
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Received: 25 October 2013
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