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| Effects of Anti-stress Agents on Serum Biochemical Parameters and Hepatic Antioxidant Parameters in Wuchang Bream (Megalobrama amblycephala) during Long-distance Transportation |
| ZHOU Chuan-peng,LIU Bo,XIE Jun,GE Xian-ping,XU Pao,WANG Ke-bao,ZHOU Qun-lan,PAN Liang-kun,CHEN Ru-li |
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Abstract The serum cortisol (COR) ,levels of 3 ,5 ,3′ - triiodothyronine (T3) ,complement 3 (C3) , complement 4 (C4) ,hepatic malondialdehyde (MDA) ,activity of superoxide dismutase (SOD) ,and total antioxidant capacity (T-AOC) were determined in Wuchang bream (Megalobrama amblycephala) with initial body weight of (600 ± 12) g held in 1m3 tanks of 750 fish fed the diets containing 20 mg/L vitamin C , glutamine or anti-stress compund (anti-stress agents ) (without addition as control group ) during long-distance transportation period of 0 h ,4 h ,8 h ,and 12 h to evaluate the effect of anti-stress agents on the serum biochemical parameters and hepatic antioxidant abilities during the long-distance transportation period .The results showed that the anti-stress agents led to significantly (P<0 .05) decrease in serum cortisol levels and hepatopancreas MDA levels ,and to significantly (P <0 .05) increase in serum C3 ,C4 , hepatic SOD ,and T-AOC compared with the control group ,indicating that the anti-stress agents was used to regulate biochemical parameters and antioxidant ability .Based on the serum levels of COR ,T3 ,C3 , C4 ,and hepatic MDA ,SOD activity ,and T-AOC ,the antioxidant ability order of the anti-stress agents was ranged as vitamin C>glutamine>anti-stress compound .
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Received: 25 January 2014
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