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| Effects of Hg on Three Defense Related Enzyme Activities in Early Developmental Stages of Pacific Oyster Crassostrea giga |
| LIANG Jian-guang,BAI Xue-feng,GONG Qi,FU Zheng-yang,SUN Lei,WANG Yi-yan |
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Abstract The effects of heavy metal Hg on three defense related enzyme activities were investigated in embryos and larvae of Pacific oyster Crassostrea giga to elucidate the effects of heavy metals on physiological and biochemical indexes and toxic mechanism during early developmental stages of bivalves . The results showed that the acid phosphatase (ACP) and alkaline phosphatase (ALP) activities were found to be reduced significantly (P<0 .01) in embryos and larvae exposed to Hg concentrations of 3 μg/L ,30μg/L and 300 μg/L for 2 h (morula stage) ,5 h (gastrula stage) ,8 h (trochophore larval stage) ,and 20 h (D-shaped veliger larval stage) compared with those in control group .The peroxidase (POD) activities of embryos and larvae were also found to be inhibited significantly (P<0 .05) in the groups of 3μg/L and 30μg/L compared with the control group ,while the POD activity were increased in 30 μg/L group (P<0 .01) .In control group ,three enzyme activities were changed with the development of Pacific oyster , with increases in activity in morula stag ,then decrease ,the minimum in gastrula and trochophore stages and increase in veliger larvae .The findings indicate that the toxic effects of metal on embryos and larvae of bivalves might be involved in the changes in ACP ,ALP and POD activities .
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Received: 25 January 2014
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