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| The Anesthesia Effects of Clove Oil on Koi Carp (Cy prinus carpio) |
| ZHANG Zhi-ming,HU Pan,JIANG Zhi-qiang,LIU Dong-chao,DING Hui-ping |
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Abstract T he effects of clove oil (10 mg/L ,30 mg/L ,60 mg/L ,100 mg/L and 200 mg/L ) on anesthesia was investigated in koi carp (Cyprinus carpio) with body length of 12-15 cm and body weight of 30-40 g at water temperature of (18-20 ℃) .The anesthesia and recovery process were both divided into five stages according to the characteristics of the fish behavior .The anesthesia induction period was found to be shortened from 32 .27 min at 30 mg/L of clove oil to 4 .10 min at 200 mg/L of clove oil ,w hile the recovery time was found to be significantly prolonged from 8 .26min at 30 mg/L of clove oil to 13 .38 min at 200 mg/L of clove oil (P< 0 .01) .The recovery rate were both 100% in the fish exposed to 30 mg/L clove oil solution for 60 min and 60 mg/L clove oil solution for 30 min ;60% in the fish exposed to 100 mg/L for 20 min ;80% in the fish exposed to 200 mg/L for 10 min and 20% for 15 min .The fish exposed to air after deep anesthesia in clove oil solution had the moving time of 2 .1 min at 30 mg/L of clove oil and prolonged gradually to 25 .8 min with the increase in clove oil concentration up to 200 mg/L (P< 0 .05) .The recovery rate in the fish exposed to different concentrations of clove oil for 24 h was 0% in 35 mg/L ,60%in 30 mg/L and 100% in 10 mg/L ,15 mg/L ,20 mg/L and 25 mg/L .T he oxygen consumption rate and ammonia excretion rate of the koi carp were both found to be significantly declined with the elevated concentration of clove oil from 5 mg/L to 25 mg/L (P<0 .05) .It is suggested that 15-20 mg/L of clove oil be of optimal dose for transportation ,and 100 mg/L of clove oil be of optimal dose for handling .
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Received: 25 January 2014
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