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| Development of a Liquid Chip Technique for Simultaneous Detection of Taura Syndrome Vi rus (TSV)and Yellow Head Disease Vi rus (YHDV) |
| YIN Wei-li,ZHANG Si-hua,YUE Zhi-qin,ZHENG Xiao-long,LIU Hong |
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Abstract The CP2 gene of Taura syndrome virus (TSV) and N gene of yellow head disease virus(YHDV) in the GenBank were analysed by the software DNAStar 7 .0 ,and specific TSV and YHDV primers labeled with biotin were prepared and coupled with fluorescence-coded microspheres to develop liquichip technique to detect TSV and YHDV simultaneously .The aminationed probe was used for hybridization reaction to PCR products of TSV and YHDV ,and then the liquichip detection technique for detection of TSV and YHDV was established using BD FACSArray to detect fluorescence signal in the reaction system .This assay was highly sensitive to TSV ,and YHDV with a lower detection limit of 100 pg ,and was not susceptible to cross reaction with other viruses ,including white spot syndrome virus (WSSV ) ,spring viremia of carp virus (SVCV ) ,and infectious haematopoietic necrosis virus (IHNV ) .In conclusion ,the liquichip technique assay is sensitive ,and specific for TSV and YHDV detection ,and provides a novel , convenient ,and rapid approach for the detection of TSV and YHDV .
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Received: 25 January 2014
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