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| Effects of Dietary Carbohydrate Levels on Immunity and Serum Biochemical Indices under Low Temperature Stress in Tilapia (Oreochromis niloticus) |
| LIU Kang,HUANG Kai,QIN Xi,WU Hongyu,CHENG Yuan,HUANG Qing,HUANG Xiuyun |
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Abstract A feeding trial was conducted to investigate the effect of immune and serum biochemical response of tilapia (Oreochromis niloticus) juveniles under low temperature (9 ℃)stress .Diets with eight carbohydrate levels (10% ,15% ,20% ,25% ,30% ,35% ,40% ,and 45% ) were fed to tilapia juveniles twice a day for 49 days .The oxidation resistance levels was found to be increased at first and decreased subsequently with increasing dietary carbohydrate levels .The maximal lysozyme activity of 54 .08 U/100 mL was observed in the fish fed the diet containing carbohydrate level of 35% . The total superoxide dismutase was increased with increasing dietary carbohydrate levels .The tilapia fed the diets containing 35% carbohydrate showed significantly (P <0 .05) higher catalase value than the tilapia fed diets containing 10% , 40% and 45% carbohydratedid .Under low temperatures stress ,the liver and cardiac muscles of the fish fed the diet containing high levels of carbohydrates were damaged ,resulting in abnormal serum biochemical indices .
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Received: 25 February 2014
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