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Phosphatase Activity and Effects of m-Cresol on Phosphatase Activity during Early Development of Common Carp Cy prinus carpio var .color |
ZHU Jun-hua,YAO Jun-jie,FENG Ya-nan,JIANG Zuo-yu,XIONG Hua-long |
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Abstract Activities of acid phosphatase ( ACP) and alkaline phosphatase ( ALP) were determined and the effect of m-cresol on the activities of ACP and ALP was studied during early development of common carp Cyprinus carpio var .color by biochemical and static toxicological methods to evaluate the toxic effect of environmental estrogens on early development of fish . The results showed that the activities of phosphatase were lower in the fertilized eggs than those in the mature eggs ,with the minimal value in cleavage stage of the fertilized eggs exposed to the m-cresol at concentration of 7 mg/L ,13 mg/L and 19 mg/L at water temperature of (20 ± 2) ℃ .There was a close dose-effect and development stage-effect relationship for the activities of ALP and ACP in each concentration group .In general ,the activities of ALP and ACP were found to be increased with the development and the increase in m-cresol concentration during different early development stages of the common carp .
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Received: 25 February 2014
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