|
|
|
| Microsatellite Analysis of Genetic Characteristics in Spotted Mandarinfish Siniperca schezeri♀ × Mandarinfish S .chuatsi♂Hybrids |
| LI Chuanyang,XU Miaoyang,ZHAO Jinliang,QIAN Yezhou,QIAN De,WU Chao |
|
|
|
|
Abstract The genetic characteristics of spotted mandarinfish Siniperca scherzeri ♀ × mandarinfish S .chuatsi ♂hybrids F1 , and F2 were analysed by 12 selected species-specific microsatellite markers . The results showed that there wer effective number of alleles (Na) of 2 .917 ,observed heterozygosity (Ho) of 0 .440 , and polymorphism information content ( PIC) of 0 .450 in spotted mandarinfish , higher than those in mandarinfish (Na=2 .167 ,Ho=0 .310 ,and PIC=0 .388) .The higher heterozygosity was observed in F1 and F2 than that in spotted mandarinfish and mandarinfish .Heterozygous genotypes at each locus were presented in F1 ,indicating that F1 is a true diploidy hybrid .The genetic distance between F1 and spotted mandarinfish (0 .3446) was larger than that in mandarinfish (0 .2401) ,and F1 was found to be inclined to mandarinfish in genetic phenotype .The minimal genetic distance was found between F2 and F1 ,and some genetic segregation were observed at 10 loci .These findings would provide some molecular data for genetic identification of hybrids and breeding of Siniperca species .
|
|
Received: 25 February 2014
|
|
|
|
|
|
|
