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| Genetic Diversity and Differentiation of Eelgrass Zostera marina along Yantai and Weihai Coasts by Simple Sequence Repeat (SSR), in Reference to Conservation and Remediation of Seagrass Bed |
| TIAN Ping-ping,LI Xiao-jie,ZHANG Li-nan,JIANG Xin,PAN Jin-hua,YU Shen-hui,PENG Jie |
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Abstract Seventeen pairs of Simple Sequence Repeat (SSR) primers selected from 26 primers in a total of 57 individuals of eelgrass Zostera marina were applied to study genetic diversity and genetic differentiation in 3 populations of the eelgrass along Yantai and Weihai coasts (Chudao Peninsula ,RZM ;Danzi Island , DZM ;Gulf of Yudai Hill ,YZM) .The 17 loci were all found to be polymorphism ,and the alleles (Na) had 120 in number ,with an average of 7 alleles per locus .There were average effective number of alleles (Ne) of 2 .30 ,expected heterozygosity (He) of 0 .4743 ,observed heterozygosity (Ho) of 0 .4128 and Nei’ s gene diversity (H) of 0 .4700 ,the maximal genetic diversity in RZM population ,followed by YZM and the minimum in DZM population .The value of Fst (the average of genetic differentiation) was ranged from 0 .0534 to 0 .1226 and Nm (gene flow ) from 1 .7895 to 4 .4344 among the 3 populations ,indicating that the genetic differentiation may be attributed to meadow fragmentation in history .The AMOVA (analysis of molecular variance) showed that there was 89 .51% of genetic variation within populations ,and 10 .49%among populations . The result of UPGMA (unweighted pair-group method with arithmetic means ) dendrogram of the 3 seagrass populations was found to be consistent with results of the genetic distance among them .Overall ,the relatively lower genetic diversity in YZM population implied that the population needed sufficient conservation and remediation to increase the adaptive capacity to environmental changes and evolutionary potentials .The relatively lowest genetic diversity in DZM population was found to be attributed to the unsuitability of local substrate .Otherwise ,RZM population showed relatively higher genetic diversity and differentiation ,w hich is supposed to provide materials for transplantation of seagrass and supplement of seeds to other population in Yantai and Weihai coasts .
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Received: 25 February 2014
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