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| Development of a Liquid Chip Technique for Simultaneous Detection of Red Sea Bream Iridovirus (RSIV)and Koi Herpesvirus (KHV) |
| YIN Weili,GENG Jinpei,LIU Ning,YUE Zhiqin,ZHENG Xiaolong,LIU Hong,XU Hongyan |
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Abstract The CP gene of Red Sea Bream Iridovirus (RSIV) and TK gene of Koi Herpesvirus (KHV) in the GenBank was analysed using the software DNAStar 7 .0 ,and specific RSIV and KHV primers labeled with biotin was prepared and coupled with fluorescence-coded microspheres to develop liquidchip technique to detect RSIV and KHV simultaneously .The aminationed probe was used for hybridization reaction to PCR products of RSIV and KHV ,then Liquidchip detection technique for detection of RSIV and KHV was established by BD FACSArray to detect fluorescence signal in the reaction system .The results showed that RSIV and KHV was accurately and high specifically detected by liquidchip detection technique with minimal limit of 10 pg of RSIV DNA and 100 pg of KHV DNA .The Liquidchip detection technique for detection of RSIV ,and KHV was established preliminarily ,which provided a foundation for further research on rapidly high throughout detection of other aquatic animal pathogens .
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Received: 25 March 2014
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