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| The Relative Expression of Four Stress Biomarker Genes in Hepatocytes of Atlantic Salmon (Salmo salar L .) Fed Diets with Replacement of Fish Oil with Vegetable Oil |
| ZHOU Ji-shu,STUBHAUG Ingunn,KRVEL Anne,OLSVIK Pl A,TORSTENSEN Bente E,JI Hong |
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Abstract A total of 100 individuals of juvenile Atlantic salmon (Salmo salar L .) with body weight of(165 ± 15)g were divided equally into two groups and fed diets containing fish oil or soybean oil for 5 months at water temperature of 10 ℃ and a salinity of 32-34 .The fatty acid composition was analyzed in the livers and the hepatocytes was isolated and cultured for 24 h in 3 individuals sampled randomly from each group . Then total RNA in the cells was extracted and the relative expression of stress biomarker genes of glutathione peroxidase (GSH-Px) ,heat shock protein 70 (HSP 70) ,metallothionein (MTA) and ubiquitin was studied by RT-PCR to evaluate the effect of dietary fish oil (FO) and vegetable oil (VO) on stress in Atlantic salmon .The results showed that the fatty acid composition of the liver was affected by different oil based diet while the growth was not found significantly different between the two groups (P>0 .05) . There was very significantly higher relative mRNA expression of the four stress biomarkers in the hepatocyes of the fish fed the diet containing VO than the fish fed the diet containing FO (P< 0 .01) , indicating that VO induced higher stress state for Atlantic salmon .The findings provided the valuable information for the feasibility of replacing dietary FO with VO in Atlantic salmon diet .
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Received: 25 April 2014
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