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| Effects of Fresh Epigeic Earthworm (Eisenia f oetida) Supplement on Digestive Enzyme Activity in Hybrid Snakehead |
| WANG Chao,MAO Shen-li,ZHOU Ai-guo,YAO Dong-lin,CHEN Jin-tao,ZOU Ji-xing |
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Abstract Hybrid snakehead (Channa argus ♂ × C .maculata♀) was fed diets supplemented with various levels (0% ,5% ,7 .5% ,and 10% )of fresh epigeic earthworm (Eisenia f oetida) for 100 d to investigate the effects of earthworm supplementation on digestive enzyme activities in hybrid snakehead .The results showed that there was significantly higher amylase activity in stomach in the fish fed the diets supplemented with fresh earthworm than that in the fish in the control group (P<0 .05) ,the maximum in the fish fed the diet containing 7 .5% earthworm .The amylase activities in the hepatopancreas and pyloric caeca were found to be higher in the fish fed the diets supplemented with fresh earthworms than that in the fish in the control group , without significant difference ( P > 0 .05 ) . The trypspin activities in the hepatopancreas , foregut and mid-gut were shown to be increased with the increase in earthworm supplementation levels ,significant difference between 10% earthworms addition group and the control group (P<0 .05) ,and the maximum trypspin activity in the pyloric caeca in 7 .5% earthworm addition group and significantly higher than in other groups (P<0 .05) .For the pepsin ,the earthworm showed little effect on the activity in all digestive tissues ,especially in the stomach . The activity of lipase in hepatopancreas and mid-gut was found to be increased with the increase in earthworm supplementation levels ,significantly higher in 7 .5% and 10% earthworm addition groups than that in the control group (P<0 .05 ) , and no significant differences in the midgut among each group . With the increase in the earthworm supplementation ,the lipase activity in the stomach was found first increase and then decrease , significantly higher in the 5% and 7 .5% earthworm addition groups than in the control group (P<0 .05) . In the pyloric caeca ,there was significantly higher activity of lipase in 7 .5% earthworm addition group than in the control group (P<0 .05) .
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Received: 25 April 2014
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