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| Effects of Quizalofop-P-ethyl · Benazolin on Expression of Esterase Isozymes in Different Tissues of Orential Weatherfish Misgumus anguillicaudatus |
| YU Shu-chi,ZHU Jing-jing |
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Abstract The effect of Quizalofop-P-ethyl · Benazolin on the expression of esterase (EST ) isozymes in five tissues including heart ,hepatopancreas ,spleen ,gill ,and muscle of orential weatherfish Misgurnus anguillicaudatus with body weight of 25 .20~28 .06 g were studied at water temperature of 23~25 ℃ by vertical polyacrylamide gel electrophoresis . The results showed that various numbers of EST strips showing different strength were observed in the different tissues of the orential weatherfish exposed to different concentrations of Quizalofop-P-ethyl · Benazolin for 24-96 h .The order of activity in tissues was arranged as :spleen > gill> heart > muscle > hepatopancreas .The EST expression was found to be increased in 24 h ,showing high-level of excitability ,and then decreased in 48 h or 72 h ,indicating that the expression was inhibited ,and increased again in 96 h ,which showed its adaptability .
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Received: 25 April 2014
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