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| Extraction Process of Lectin from Laminaria j aponica |
| LI Dan-tong,LI Yue,YIN Jian-peng,LI Shuai,LI Xiao-li |
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Abstract To further probe the best extract solution of lectin from L aminaria j aponica .In this study ,we separately selsct four kinds of single factor :pH ,Material‐water ratio ,soaking time and temperature .Buffer select PBS ,TBS and mannitol ,Material‐water ratio selected six gradient (1∶5—1∶30) ,soaking time select six gradient (5 -30h) ,temperature select nine gradient (30 -110 ℃) ,which adopt a method of controlling the three factors being equal ,to get the best extraction effect of each factor .Optimum results are buffer of TBS pH 7 .7 ,Material‐water ratio of 1∶30 ,soaking time for 15h and temperature of 40 ℃ and 60 ℃ .Elected from each single factor that the optimal conditions of extraction and two adjacent levels (three levels ) of it ,to make the four factors and three levels of orthogonal test . According to the orthogonal test range analysis ,buffer of TBS pH 7 .7 ,Material‐water ratio of 1∶20 ,soaking time for 20h , the temperature of the 50℃ is this best combination of each factor for extracting lectin from L aminaria j aponica .We get an optimum combination for extraction of lectin from L aminaria j aponica ,make the extraction process has been optimized .It provide a favorable theoretical basis for research of lectin from L aminaria j aponica .
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Received: 25 May 2014
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