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| Identification and Changes in Membrane Fatty Acid Composition at Low Temperature in Vibrio alginoly ticus from Frozen Cuttlefish Sepiella maindroni |
| LI Zheng-yi,JIA Jun-tao,CAO Ji-juan,XU Biao,JIANG Ying-hui |
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Abstract Bacterial strain C6‐1 isolated from cuttlefish Sepiella maindroni was characterized and changes in membrane fatty acid composition of the bacterial strain C 6‐1 in response to low temperature were also studied . The physiological and biochemical characteristics were analyzed using VITEK 2 compact automated microbiology system . The 16S rRNA gene was sequenced and subjected to phylogenetic analysis ,and the fatty acids were detected by gas chromatography‐mass spectrometry (GC‐MS) .It was found that strain C6‐1 was Gram‐negative and susceptible to the vibriostatic agent O/129 ,that exhibited the maximal level of 98% probability to Vibrio alginolyticus based on the conventional physiological test . The sequence analysis of 16S rRNA gene of C6‐1 isolation showed that C6‐1 was very close to V . alginolyticus (GenBank No .JF836167) with similarity of 99% .The major fatty acids were shown to be C12 :0 ,C14:0 ,C16 :0 ,C16:1(n‐7) ,iso‐C17 :0 ,C18 :0 and C18 :1 (n‐9) .The major change in fatty acid composition in response to low temperature was characterized by increase in fatty acids with chain lengths less than 16 carbons from 14 .8% to 44 .1% .
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Received: 25 May 2014
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