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| Complement Gene Expression in Amphioxus Challenged with Escherichia coli and Staphy loccocus aureus |
| WANG Guang-feng,LI Fang,ZHANG Shi-cui |
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Abstract The expression of complement key genes Bf ,C3 ,C6 and MASP was investigated in RNA extracts from gut and gutted body in amphioxus challenged with Escherichia coli and Staphyloccocus aureus by a real‐time PCR assay and specific primers designed for selected genes at 1 ,3 ,12 ,24 ,36 ,48 and 72 h post‐infection (pi) with E .coli and S .aureus .The results showed that the patterns of the gene expression were similar in the amphioxus challenged with both bacteria .Compared with the controls ,Bf , C3 and C6 genes showed a similar up‐regulation profile after challenge .In contrast ,MASP expression was found to be decreased significantly after the amphioxus was challenged with both bacteria .The expression of Bf ,C3 and C6 genes was significantly up‐regulated and peaked earlier in the gut than in the gutted body .T he findings indicate that the complement components play an important role in the acute phase of infection and that amphioxus digestive system rich in mucosa may be the major immune tissue responding to bacterial challenge and play an important role in the immune response .
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Received: 25 May 2014
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