|
|
|
| Embryonic,Larval and Juvenile Development of Hybrid between Tawny Puffer Taki f ugu f lavidus (♀) × Redfin Puffer Taki f ugu rubripes (♂) |
| ZHANG Fu-chong,ZHAO Hai-tao,WAN Yu-mei,FANG Xiao |
|
|
|
|
Abstract In this paper ,interspecies hybridization between tawny puffer Takif ugu f lavidus (♀ ) and redfin puffer T .rubripes (♂) were carried out by ecological adjustment and control ,hormone induction and artificial propagation . The development of the hybrid embryonic , larval and juvenile was also observed ,and morphological characteristics and duration were described in detail .The fertilized eggs as an adhensive demersal egg were measured (1 .02 ± 0 .04) mm in diameter ,and hatched on about the 150th hour after fertilization of the hybrid , and the embryonic development had total effective accumulated temperature of 2790 ℃ at water temperature 18 ~ 19 ℃ and a salinity of 30 ~ 32 . The embryonic development was divided into 7 stages ,and 24 substages ,and the newly hatched larvae had total length of (2 .52 ± 0 .15) mm ,with a large yolk sac ,accounting for about one third of the body .The myomere and systemic circulation were clearly observed in the freshly hatched larvae whose yolk sac was almost exhausted four days later .The larvae began exogenous feeding and completely fed 8 days later .After 15 days ,the front part of the larval belly was covered with a large number of “tiny prickles” and ‘air‐blowing’ was found in the larvae . After 18 days , the larvae were developed into juveniles with tooth formation .After 25 days ,the juveniles had the front teeth ,showing attacking behavior each other with mortality peak .After 41 days ,the juveniles developed into subadult ,more similar to tawny puffer ,and showed morphological characteristics in the early stage of the hybrids .
|
|
Received: 25 June 2014
|
|
|
|
|
|
|
