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| The Anaesthetic Effect of MS-222 on Megalobrama pellegrini Juveniles |
| QU Huan-tao,LIU Yong,GUO Wen-tao,XIAO Kan,GUAN Min,LI Sha,LU Xue-bao |
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Abstract The anaesthetic effect of MS‐222 on Megalobrama pellegrini juveniles was studied in water tanks in a laboratory at water temperature of 24—25 ℃ .In terms of the behavior characteristic of the fish , the anaesthetia was divided into five stages ,and the recovery process was divided into four stages .The fish was found to be anaesthetized more rapidly with increase in the concentration of MS‐222 (F=430 .263 ,P< 0 .01) ,with effective dose of 70—120 mg/L for anaesthetia . The fish showed rapid increase in respiratory frequency during the anaesthetic stage 1 and stage 2 first ,and then decline slowly from stage 2 to stage 4 ,until the fish stopped opercula moving in the stage of 5 due to excessive anaesthetia .There was a positive correlation between exposure time to air and recovery time for deeply anaesthetized fish (F=145 .416 ,P < 0 .01) ,significant difference(P<0 .01) among different exposure time groups except the two shorter exposure groups(3 min and 4 min) .The fish had recovery rate of 100% when the fish was exposed to MS‐222 within 5 min ,and only a few fish was recovered over 5 min .The fish came to death when the fish was exposed to MS‐222 for 12 min .
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Received: 25 June 2014
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