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| Toxic Effects of Cupric Acetate and Avermectins on Orange Spotted Grouper Epinephelus coioides |
| CHENG Min-hong,YANG Xiao-li,PANG Qiang,HUANG Jian-rong,LI Zu-fu |
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Abstract In this study ,the activities of acetylcholinesterase (AChE) ,superoxide dismutase (SOD) ,and aspartate transaminase (GOT ) and glutathione (GSH ) content were studied in liver of orange spotted grouper Epinephelus coioides exposed to cupric acetate and Avermectins at various concentrations to evaluate acute toxicity of cupric acetate and Avermectins .The results showed that the 24 h LC50 and 48 h LC50 were 103 .51 mg/L and 50 .86 mg/L in cupric acetate and 0 .02543 mg/L and 0 .01726 mg/L in Avermectins , with safe concentration of 3 .684 mg/L for cupric acetate and 0 .00239 mg/L for Avermectins .Cupric acetate and Avermectins stress resulted in significant changes in AChE ,SOD ,and GOT activities in liver (P< 0 .05) ,whereas Avermectins showed insignificant effects on GOT activity compared with control group . The AChE , SOD , and GOT activities were primarily induced at low concentration of cupric acetate and Avermectins in short term exposure ,and inhibited at high concentration of cupric acetate and Avermectins in long term exposure , with significantly ( P < 0 .05 ) at higher concentration .The lower liver GSH contents were in 2 mg/L ,4 mg/L and 8 mg/L groups than that in the control group in 48 h cupric acetate exposure(P>0 .05) ,but higher than that in the control group in 96 h cupric acetate exposure (P< 0 .05) .There were significantly lower liver GSH contents in 2 .5μg/L ,4 .25μg/L ,7 .25 μg/L and 12 .25 μg/L Avermectins groups than that in the control group . The findings indicate that cupric acetate and Avermectins stress affect AChE , SOD and GOT activities and GSH content ,and that biological effects of pesticides on orange spotted grouper are reflected in changes in activities of AChE ,SOD and GOT and GSH content .
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Received: 25 August 2014
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