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| Isolation,Identification and Immobilization of Photosynthetic Bacteria from Chinese Soft Shelled Turtle Triony x sinensis Culture Waters |
| ZHU Ling-yu,DAI Ai-min,DONG Ming-qian,YANG Shuo,GUAN Yue-qiang |
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Abstract A strain of photosynthetic bacterium was isolated from the “red water” in Chinese soft shelled turtle Trionyx sinensis culture ponds ,and were identified and immobilized by morphological ,physiological and biochemical characteristics and 16S rDNA sequencing .It was found that the bacterium with dull red round and regular colonies was short rod-shaped ,gram-negative ,motile ,and cultative anaerobic ,and i-dentified as Rhodobacter sphaeroides .The immobilized material screening showed that good quality cross-linked microsphere was observed in 3% sodium alginate solution with 4% CaCl2 solution .The Chinese soft shelled turtle culture water treated by immobilized R .sphaeroides in a laboratory modulated experiment showed that 42 .2% of COD and 43 .1% of ammonia nitrogen were eliminated from the waters in free cells group and 65 .1% of COD ,and 62 .1% of ammonia nitrogen in the immobilized cells ,indicating that immo-bilization of the bacteria improves the treatment effect of aquaculture waste waters .The findings will pro-vide reference to the treatment of wastewater produced by Chinese soft shelled turtle or other cultured ani-mals .
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Received: 25 August 2014
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