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| Influence of Maternal Immunization on Expression of Lysozmye Genes in Zebrafish Embryos |
| LIU Xiao-ning,LI Zheng-long,WANG Zhi-ping,LI Ya-lin |
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Abstract Maternal immunity ,the immunity transfered from mother to offspring ,plays a key role in pro-tection of the vulnerable offspring against pathogenic attacks during early development stages of fish .T he influence of mother immunization on the expression of lysozyme in zebrafish embryos was studied here , with the aim to demonstrate that mother immunization can improve the vertical transfer of maternal immu-nity and then improve the immunocompetence of the offspring .The mother zebrafish was immunized with the formalin-killed Aeromonas hydrophila ,the embryos were then collected every 7 days (zebrafish repro-duced eggs weekly in appropriate conditions ) ,total RNA was extracted and cDNA was synthesized with reverse transcription .The expression of maternal lysozyme was determined by real-time quantitative PCR . The results showed that mother immunization improved the expression level of maternal lysozyme in the newborn embryos ,the peak in 2 weeks .The findings indicate that vertical transfer of maternal immune factors at gene level (mRNA) should be a effective way for the maternal immunity ,and that mother immu-nization can improve the vertical transfer of maternal immunity .
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Received: 25 August 2014
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