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| Effects of Rehmannia glutinosa and Small Peptide on Growth and Expression of Interleukin-8 in Common Carp Cy prinus carpio |
| WANG Jun-li,LUO Yan-ting,HAO Guang,YAN Xiao,WU Chun,NIE Guo-xing |
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Abstract The effects of Rehmannia glutinosa and marine source bioactive small peptide on growth and im-mune function in common carp (Cyprinus carpio) were investigated to develop Rehmannia glutinosa and the small peptide as fish feed additives .In Experiment 1 ,540 common carp juveniles with body weight of (7 .46 ± 1 .06) g were randomly allocated into nine groups ,and fed basal diet (control group) ,the diets containing radix rehmanniae pow der at a dose of 2% and 4% ,the diets containing prepared rehmannia root powder at a dose of 2% and 4% ,the diets containing radix rehmanniae extract at a rate of 0 .5% ,and 1% , or the diets containing prepared rehmannia root extract at a dose of 0 .5% and 1% for 80 days .In Experi-ment 2 ,360 common carp juveniles with body weight of (5 .47 ± 0 .3) g were randomly allocated into four groups ,and fed the basal diet (control group ) ,and the diets containing small peptide at a rate of 5% , 10% and 15% for 80 days .At the end of the experiments ,the growth performance ,disease resistance and the IL-8(interleukin-8) mRNA expression in spleen were studied in the juveniles .The results showed that the higher expression of IL-8 in the spleen was found in the juveniles fed the diets containing all sorts of re-hmannia preparations and different doses of the small peptide ,significantly higher in 2% prepared rehman-nia root powder ,4% prepared rehmannia root powder ,1% radix rehmanniae extract ,0 .5% prepared reh-mannia root extract and 5% and 10% small peptide groups than those in the control groups ,indicating that Rehmannia glutinosa can significantly promote the growth and disease resistance of common carp .Therefore ,Re-hmannia glutinosa and marine source bioactive peptide are expected to become good fish feed additives .
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Received: 25 September 2014
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