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| Effects of Lactobacillus on Immunity and Disease Resistance of Tilapia (Oreochromis niloticus × O . aureu) |
| HUANG Yan-hua,ZHOU Xiao-bo,WANG Guo-xia,KUANG Zhe-shi,ZHAO Hongxia,MO Wenyan,CAO Junming |
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Abstract The experiment was conducted to study the effects of homology Lactobacillus preparation with different strains in diets on immune ,antioxidant and resistance against Aeromonas hydrophila .The tilapia hybrid (Oreochromis niloticus × O . aureu) with initial body weighg of 3 .49 g were fed the basal diet in control group ,and fed the basal diet containing L .acidophilus ,L .plantarum ,L .pentosus ,L .rhamno-sus and Enterococcus f aecalis in the other five groups for 8 weeks .The number of live lactic acid bacteria in each L actobacillus used in the experiment was 1 .30 × 103 ,5 .10 × 108 ,2 .97 × 108 ,3 .03 × 108 ,2 .30 × 108 and 3 .47 × 108 cfu /g ,respectively .At the end of the feeding test ,the fish were infected with Aeromonas hydrophila for 72 hours .The serum acid phosphatase activity was increased in varying degrees ,the maxi-mum in L .p lantarum group ,L .pentosus group ,L .rhamnosus group and E . f aecalis group in L .pento-sus group the highest was ,significantly higher than that in L .acidophilus group .The serum antisuperox-ide anion free radical activities were increased significantly ,higher in L .rhamnosus and E .f aecalis groups compared with the others .The activity of serum lysozyme was significantly higher in L .rhamnosus group than in the control group .During injection of A .hydrophilain 12 h ,the survival rate was higher in Lacto-bacillus groups than in the control ,no significant difference .In 24 and 48 hours ,survival rate were sig-nificantly higher in the L . rhamnosus and E . f aecalis groups than control group .In 72 hours ,survival rate in the L .rhamnosus group was significantly higher than control group .The results suggested that the supplementation of L actobacillus into the practical diet enhanced anti-oxidation ,and immune capacity and improved the resistance against A .hydrophila in tilapia .
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Received: 25 October 2014
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