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| Optimization and Analysis of Two-dimensional Electrophoresis of Hepatopancreas Proteins in Chinese Shrimp (Fenneropenaeus chinensis) under Crowding Stress |
| ZHANG Shuai,FU Ling-lin,WANG Yan-bo |
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Abstract The two-dimensional electrophoresis (2-DE) was optimized and differentially expressed proteins were investigated in hepatopancreas of Chinese shrimp (Fenneropenaeus Chinensis) under crowded condi-tion .The results showed that the optimal two-dimensional electrophoresis were observed under the condi-tions of addition of TBP (tributyl phosphate) ,strips of 17 cm ,and pH 4-7 IPG (Immobilized pH gradi-ent) ,loading the sample of 120 μg and setting the voltage and power of isoelectric focusing as 65 000 Vhr . The relative 2-DE maps showed that approximate 600 spots was detected in each gel ,and a total of 11 dif-ferentially expressed protein spots were found ,including 8 significantly up-regulated protein spots (P <0 .05) and 3 significantly down-regulated protein spots (P < 0 .05) .The findings indicated that proteins in the shrimp hepatopancreas were differently expressed under crowding stress .
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Received: 25 October 2014
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