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| Intestinal Flora in Supply Chain of Live Released Yesso Scallop Pationopecten yessoensis |
| LIU Shi-yong,LIU Jun-rong,MA Yong-sheng,SHEN Jian,YANG Ting-ting,MIAO Zhi-ou |
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Abstract The similarity of intestinal flora with growing environment and changes in flora structure were studied in yesso scallop Pationopecten yessoensis in live supply chain ,including 6 key links from post cap-ture on board ,live transport ,tranship ,depuration Ⅰ ,depuration Ⅱ and marketing .The scallop ,seawa-ter and sediments on deck of scallop-dredging vessels were collected ,and the scallop was also sampled a-long the supply chain at each link .The flora in the scallop intestine ,seawater and sediment samples was analyzed by 16S rDNA PCR-DGGE (denaturing gradient gel electrophoresis ) fingerprint . The results showed that 12 bands were observed in the intestine ,18 bands in the sediments and 20 bands in the seawa-ter ,including 9 bands being shared .There were 14 bands live transport ,16 bands in tranship ,13 bands in depuration Ⅰ ,9 bands in depuration Ⅱ and 22 bands in markets .Similarity analysis revealed that the sim-ilarity was 66 .6% between intestinal flora and seawater flora ,and 62 .5% between the intestinal flora and sediment flora .The similarities were found to be 64% between the intestinal flora and flora in live trans-port ,73 .3% between the intestinal flora and flora in tranship ,69% between the intestinal flora and flora in depuration Ⅰ ,63 .6% between the intestinal flora and flora in depuration Ⅱ,and 32 .3% between the in-testinal flora and flora in marketing .It is concluded that DGGE fingerprint band was increased in number from capture to depuration and from post-depuration to market ,indicating that the changes in post-capture environment had influence on the flora ,and that the depuration leads to reduce in microbiological contami-nation effectively .The live scallop was in a state of stenoplastic stress from capture to depuration ,and in a state of more obvious stress from post-depuration to market ,that is ,the terminal retail link being a sig-nificant impact on the quality of live scallop .
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Received: 25 October 2014
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