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| Activities of Antioxidative Enzymes and Effects of Exogenous Vitamin C during Embryonic Development of Crucian Carp Carassius auratus |
| XIONG Hua-long,YAO Jun-jie,AN Miao,WANG Jin-na,JIANG Zuo-yu |
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Abstract T he activities of superoxide dismutase (SOD ) ,catalase (CA T ) and glutathion peroxidase and effects of exogenous vitamin C on these enzymatic activities were studied during embryonic development of crucian carp Carassius auratus at water temperature of 24 ℃ .The results showed that there were activities of SOD[(10 .213 ± 0 .458) U/mg] ,CAT [(8 .701 ± 0 .371) U/mg] and glutathion peroxidase [(55 .485 ± 4 .896) U/mg] ,being derived from maternal antioxidant enzymes .In the control group ,the activities of SOD and glutathion peroxidase were increased gradually from mature eggs to pre-hatching ,while the activ-ity of CAT showed a tend of up-down-up ,the minimum [(6 .175 ± 0 .532) U/mg] in heart beating stage , and then elevated .There were higher activities of SOD ,CAT and glutathion peroxidase from mature egg to pre-hatching in the embryos exposed to vitamin C solution at a rate of 30 mg/L than that in the control group .Maternal antioxidative enzymes was found to play an important role during embryonic development of the crucian carp and appropriate concentration of vitamin C in the water used during the embryonic de-velopment led to improve the antioxidant capacity in the embryos of the crucian carp .
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Received: 25 October 2014
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