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| Relationship between Nucleic Indicators and Growth in Larvae of Japanese Flounder Paralichthys olivaceus |
| TONG Xue-hong,WANG Huan-li,GE Bao-ming,YANG Xiao-lan,ZHANG Zhen-ming |
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Abstract Total length (L) and body weight (m) ,concentrations of nucleic acid ,and protein ,RNA/DNA ratio (a) ,and protein/DNA ratio (b) were measured in larvae of Japanese flounder Paralichthys oliv aceus by whole homogenate method and by electron microscope to evaluate the relationship between nucleic indi-cators and growth in larval Japanese flounder .It was found that body weight and total length were nega-tively correlated with RNA/DNA ratio ,with an equation as m= -19 .85 a + 82 .79 (r2 = 0 .89 , P<0 .01) ,and as L= -7 .41 a + 33 .53(r2 = 0 .85 , P<0 .05) ,indicating that the grow th of Japanese floun-der larvae was primarily involved in hyperplasia and in exhaustion of protein synthesized during the early developmental stage .Body weight and total length were also negatively correlated with protein :DNA rati-o ,with an equation m= -0 .61b + 34 .54 (r2 = 0 .93 ,P<0 .01) and L= -0 .22b + 15 .33 (r2 = 0 .86 ,P<0 .01) .There was larger decrease scope in the protein/DNA ration than that in the RNA/DNA ratio as the larvae elapse in culture .The findings suggest that nucleic indicators be used to evaluate growth rates of larval Japanese flounder on a cellular level .
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Received: 25 October 2014
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