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| Rapid Detection of Pathogen Vibrio chagasii from Abscess Yesso Scallop by PCR Technique |
| TENG Wei-ming,LI Wen-ji,ZHANG Ming,LI Shi-lei,LIU Xiang-feng,FU Cheng-dong,LIU Zhong-ying |
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Abstract The PRC primer was designed according to the hypervariable region sequence of GyrB gene in V ibrio chagasii in GenBank and applied to amplify a 144 bp DNA fragment in order to develop an effective method for the identification of V .chagasii in yesso scallop Patinopecten yessoensis diseased with abscess . The primer showed sensitivity of 0 .43 pg with detection limit of 3 .9 × 10-3 cfu ,and the PCR amplification was specific for the extracted DNA from V .chagasii ,instead of V .vulnif icus ,V .alginolyticus ,V .algi-nolyticus ,and V .splendidus .The findings indicate that the PCR offers sensitive and specific means for the detection of V .chagasii ,and the recognition of V .chagasii from diseased yesso scallop .
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Received: 25 October 2014
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