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| Genetic Variation in Mitochondrial 16S rRNA Genes in Cultured Populations of Disk Abalone Haliotis discus hannai and H .diversicolor supertexta |
| CHEN Li,SHEN Xin,XIANG Zhi-chu,QIN Yin-yin,LIN Quan-heng,M ENG Xue-ping |
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Abstract The 16S rRNA gene (16S) fragments were amplified in 53 samples of 4 populations of disk aba‐lone H aliotis discus hannai and 1 population of H . diversicolor supertexta ,and 669bp nucleotide se‐quences were used to analyze genetic differentiation among the populations of the abalone .The results showed that 17 haplotypes were detected from 52 16S sequences ,including 13 haplotypes from 4 popula‐tions of disk abalone and 4 haplotypes from 9 samples of H .diversicolor supertexta .The nucleotide se‐quences of the haplotypes revealed that 134 (account for 20 .5% ) polymorphic sites were found in 655 a‐ligned positions with 17 haplotypes .The mean intrapopulation genetic distances based on 16S was 0 .005 in disk abalone (0 .002~0 .011) and 0 .004 in H .diversicolor supertexta ,average 0 .218 between disk abalo‐ne and H .diversicolor supertexta populations .The FST was found to be 0 .892 between disk abalone and H .diversicolor supertexta ,0 .0051~0 .0065 in disk abalone population .Phylogenic analysis of 11 H ali‐otis species showed that both disk abalone and H .discus discus were clustered into single clade;and that H .diversicolor supertexta and H .diversicolor diversicolor clustered into single clade .
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Received: 25 November 2014
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