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| Immune Responses and Disease Resistance of Juvenile Sea Cucumber Apostichopus j aponicus Induced by Vibrio sp .BC 232 |
| YANG Zhi-ping,XU Zhe,ZHANG Cui-cui,ZHOU Qian |
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Abstract Vibrio sp .BC232 was added to the diets at 105 ,107and 109cfu/g .Juvenile sea cucumber A pos‐tichopus j aponicus was randomly distributed into 12 plastic tanks (100 L) at a density of 50 individuals per tank and fed one of the diets or basal diet (control) for 30 days and 45 days .Effects of V ibrio sp .BC232 on immune response and disease resistance against V .splendidus infection in sea cucumbers were analyzed after feeding experiment .Results showed that the phagocytic activity in coelomocytes of sea cucumbers fed Vibrio sp .BC232 at 107and 109cfu/g for 45 days was signicantly higher than that in the control group (P< 0 .01) .The coelomic fluid lysozyme activities were signicantly enhanced in sea cucumbers receiving Vib‐rio sp .BC232 at 105 ,107 and 109 cfu/g for 30 days ,and 109 cfu/g for 45 days ,respectively ,compared to the control group (P < 0 .05) .Sea cucumbers fed V ibrio sp .BC232 at 105 cfu/g for 30 days ,and 105 ,107 and 109 cfu/g for 45 days ,respectively ,had higher coelomic fluid phenoloxidase activities than the animals fed basal diet(P < 0 .01) .Additionally ,10 sea cucumber from each group and control group were chal‐lenged with V .splendidus NB13 after feeding for 45 days .Cumulative incidence and mortality of sea cu‐cumber fed Vibrio sp .BC232 were found to be lower than those in the control group .The results showed that diet supplemented with V ibrio sp .BC232 stimulated the immune system of juvenile thus enhancing their resistance against V .sp lendidus .
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Received: 25 November 2014
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