|
|
|
| Screening and Purification of Bacillns in Tropical Area and Effect on Artificial Wastewater |
| WU Hai-wu,GUO Cong,ZHU Yan-bo,HUANG Jie-chang,ZHOU Yong-can,GUO Wei-liang,WANG Shi-feng,XIE Zhen-yu |
|
|
|
|
Abstract A strain of Bacillus known as LS‐1305 was screened out from sediments of tropical marine aqua‐culture in Hainan province and was found to purify effectively artificial wastewater .Bacillus LS‐1305 was identified as B . f lexus by colonial morphology ,16S rDNA and biochemical characteristics tests .The LS‐1305 was inoculated at initial concentration of (2 .5 ± 0 .3)× 105cfu/mL into the artificial wastewater with initial chemical oxygen demand of (721 .5 ± 1 .8) mg/L ,ammonia nitrogen (67 .33 ± 0 .58) mg/L ,and ni‐trite (68 .56 ± 2 .08) mg/L and aerated by sterile air for 48 h to investigate the growth characteristics of LS‐1305 and safety for Pacific white leg shrimp Litopenaeus vannamei .The growth regime of LS‐1305 with time in artificial wastewater was established and the LS‐1305 was approved to be security for Pacific white leg shrimp ,with the removal efficiency of 91 .61% for chemical oxygen demand ,86 .21% for ammo‐nia nitrogen and 87 .22% for nitrite ,indicating that LS‐1305 as a microbial preparations has potential for improvement of marine aquaculture water quality and environment in Hainan .
|
|
Received: 25 November 2014
|
|
|
|
|
|
|
